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                  Xiaomei Yan  



                     President 




                                      





厦门大学化学化工学院教授,化学生物学系主任
                                               
     

        厦门大学科学仪器工程系 工学学士(1986-1990)

        厦门大学化学系 硕博连读 (1990-1996)

        中国赴日本国留学生预备学校(东北师范大学)(1993-1994)

        日本名古屋大学工学部联合培养(1994-1996)
  
        厦门大学化学系分析化学专业 理学博士 1996

        美国University of Florida化学系 博士后(1996-1997)

       美国Los Alamos 国家实验室生物科学部 博士后 (1997-2001)

       美国Los Alamos 国家实验室生物科学部 研究员 (2001-2005)

       厦门大学化学化工学院 教授、博导(2005.8-)

       厦门福流生物科技有限公司 董事长 (2014-)


       研究领域为生化分析与生物传感,致力于超高灵敏流式检测技术的发展及应用。研制成功国际首台散射和荧光同时检测的超高灵敏流式检测仪器,散射光检测灵敏度较传统流式细胞仪提升3-5个数量级,实现对单个功能化纳米颗粒以及病毒、细菌、亚细胞结构等天然生物纳米颗粒的高灵敏、高选择性、高通量检测,发展了一系列纳米生物分析新技术、新方法。已在ACS Nano, J. Am. Chem. Soc., Angew. Chem. Int. Ed., Anal. Chem.等国际主流期刊发表60多篇SCI论文,获得2项国家发明专利授权,先后主持包括国家自然科学基金杰出青年科学基金、科学仪器基础研究专款、重大研究计划培育项目、面上项目、主任基金,高等学校博士学科点专项基金,福建省科技厅重大专项前期研究项目等多个科研项目,2007年入选教育部新世纪优秀人才支持计划。2012年获得国家杰出青年科学基金资助,荣获2012年度厦门市五一劳动奖章,入选2012年度福建省高校领军人才,荣获第十二届福建青年科技奖,入选科技部2013年中青年科技创新领军人才。


Research Interests
Development of advanced instrumentation and methodologies for biochemical analysis and biosensing. 
The main research focuses include :
1) Development of high sensitivity flow cytometry (HSFCM) to address the challenge of quantitative physical and      biochemical characterization of single nanoparticles in nanotechnology, nanobiotechnology, nanomedicine,  biopharmaceuticals, and basic science.
2) High-throughput, high-resolution, and multiparameter characterization of single natural and synthetic nanoparticles such as gold, silver, silica, polymeric, and iron oxide nanoparticles, lipid-based nanomedicines, quantum dots, and viruses.
3) Specific analysis of individual bacteria for pathogen identification, antibiotic resistance detection, and biological function studies.
4) Development of advanced methodologies for the individual analysis of subcellular organelles such as mitochondria, extracellular vesicles (microvesicles and exosomes), and chromosomes.
5) Development of microsphere-based suspension array technology for biomedical and biochemical applications.
 


Publications
61. ChaoxiangChen, XiangZhang, ShuyueZhang, ShaobinZhu, JingyiXu, YanZheng, JinyanHan, Jin-ZhangZeng, XiaomeiYan. Quantification of protein copy number in single mitochondria: The Bcl-2 family proteins. Biosensors and Bioelectronics, 2015, 74, 476-482.
60. Yu, MX; Wu, LN; Huang, TX; Wang, S; Yan, XM*. Rapid detection and enumeration of total bacteria in drinking water and tea beverages by a laboratory-built high-sensitivity flow cytometer, Anal. Methods, 2015, 7, 3072-3079.
 
59. Zhang, Z*; Deng, CQ; Meng, LS; Zheng, Y; Yan, XM. A rhodamine hydrazide-based fluorescent probe for sensitive and selective detection of hypochlorous acid and its application in living cells, Anal. Methods2015, 7, 107-114.
 
58. Zhu, SB; Ma, L; Wang, S; Chen, CX; Zhang, WQ; Yang, LL; Hang, W; Nolan, J. P.; Wu, LN; Yan, XM*. Light-scattering detection below the level of single fluorescent molecules for high-resolution characterization of functional nanoparticles. ACS Nano, 2014, 8, 10998-11006.
 
57. Zhang, X; Zhang, SY; Zhu, SB; Chen, S; Han, JY; Gao, KM; Zeng, JZ; Yan, XM*. Rapid identification of mitochondria targeting anticancer compounds by an in vitro strategy, Anal. Chem. 2014, 86, 5232−5237.
 
56. Wu, LN; Luan, T; Yang, XT; Wang, S; Zheng, Y; Huang, TX; Zhu, SB; Yan, XM*. Trace detection of specific viable bacteria using tetracysteine-tagged bacteriophages, Anal. Chem. 2014, 86, 907-912.
 
55. Yang, CW; Chen, S; Zhou, M; Li, Y; Li, YF; Zhang, ZX; Liu, Z; Ba, Q; Li, JQ; Wang, H; Yan, XM*; Ma, DW*; Wang, RX*. Development of 3-Phenyl-N-(2-(3-phenylureido) ethyl)-thiophene-2-sulfonamide compounds as inhibitors of antiapoptotic Bcl-2 family proteins, ChemMedChem, 2014, 9, 1436-1452.
 
54. Xu, F; Zhu, L; Zhu, SB; Yan, XM; Xu, HC*. Electrochemical intramolecular aminooxygenation of unactivated alkenes, Chem. Eur. J., 2014, 20, 12740-12744.
 
53. Huang, ZZ; Zhang, SD; Hang, W*; Chen, YD; Zheng, JX; Li, W; Xing, JC; Zhu, EY; Yan, XM. Liquid chromatography-mass spectrometry based serum peptidomic approach for renal clear cell carcinoma diagnosis, J. Pharmaceut. Biomed., 2014, 100, 175-183.
 
52. Shao, Q; Zheng, Y; Dong, XM; Tang, K; Yan, XM*; Xing BG*. A covalent reporter of β-lactamase activity for fluorescent imaging and rapid screening of antibiotic-resistant bacteria, Chem. Eur. J., 2013, 19, 10903-10910.
 
51. Yang, LL;† Huang, TX;† Zhu, SB; Zhou YX; Jiang, YB; Wang, S; Chen, YQ; Wu, LN; Yan XM*. High-throughput single-cell analysis of low copy number β-Galactosidase by a laboratory-built high-sensitivity flow cytometer, Biosens. Bioelectron. 2013, 48, 49-56.
 
50. Chen, CX; Zhu SB; Huang TX; Wang S; Yan, XM*. Analytical techniques for single-liposome characterization. Anal. Methods, 2013, 5, 2150-2157.
 
49. Yang, L; Wu, SQ; Lin, BJ; Huang, TX; Chen, XP; Yan, XM; Han, SF*. A targetable nanogenerator of nitric oxide for light-triggered cytotoxicity. J. Mater. Chem. B, 2013, 1, 6115.
 
48. Huang, ZZ; Chen, YJ; Hang, W.*, Gao, Y.; Lin, L.; Li, D. Y.; Xing, JC; Yan, XM. Holistic Metabonomic Profiling of Urine Affords Potential Early Diagnosis for Bladder and Kidney Cancers, Metabolomics. 2013, 9, 119-129.
 
47. Zhang, SY; Zhu, SB; Yang, LL; Zheng, Y; Gao, M; Wang, S; Zeng, J-Z; and Yan, XM*. High-throughput multiparameter analysis of individual mitochondria, Anal. Chem. 2012, 84, 6421-6428.
 
46. Yang, LL; Zhou, YX; Zhu, SB; Huang, TX; Wu, LN; Yan, XM*. Detection and quantification of bacterial autofluorescence at the single-cell level by a laboratory-built high-sensitivity flow cytometer, Anal. Chem. 2012, 84, 1526-1532.
 
45. Huang, H; Chen, ZJ; Yan, XM*. Simultaneous determination of serotonin and creatinine in urine by combining two ultrasound-assisted emulsification microextractions with on-column stacking in capillary electrophoresis, J. Sep. Sci. 2012, 35, 436-444.
 
44. Lin, L; Huang, ZZ; Gao, Y; Chen, YJ; Hang, W.*, Xing, JC; Yan, XM. LC-MS based serum metabolic profiling for genitourinary cancer classification and cancer type-specific biomarker discovery, Proteomics, 2012, 12, 2238-2246.
 
43. Lu, N.; Liu, JX; Liu, J.; Zhang, CY; Jiang, FQ; Wu, H.; Chen, LQ; Zeng, WJ; Cao, XH; Yan, TD; Wang, GH; Zhou, H.; Lin, BZ; Yan, XM; Zhang, XK; and Zeng, JZ*. Antagonist effect of triptolide on AKT activation by truncated retinoid X receptor-alpha. PLoS One. 2012, 7(4): e35722.
 
42. Gao, Y.; Yang, N.; Yan, XM; Hang, W.*; Xing, JC; Zheng, J.; Zhu, EY; Huang, BL. Early diagnosis of urinary lithiasis via elementary profile of serum samples, Anal. Methods. 2012, 4, 693-698.
 
41. Wu, LN; Huang, TT; Yang, LL; Pan, JB; Zhu, SB; Yan, XM*. Sensitive and selective bacterial detection using tetracysteine-tagged phages in conjunction with biarsenical dye, Angew. Chem. Int. Ed2011, 50, 5873-5877.
 
40. Zhu, SB; Wang, S; Yang, LL; Huang, TX; Yan, XM*. Progress in the development of techniques based on light scattering for single nanoparticle detection, Sci. China Chem. 2011, 54, 1244-1253.
 
39. Zheng, Y; Lin, L; Hang, W; Yan, XM*; Marrone, B. L.* Analysis of beryllium to biomolecule binding using a metal specific fluorescent probe and competitive Assay, Talanta. 2011, 85, 638-643. 
 
38. Zhang, Z*; Zheng, Y; Hang, W; Yan, XM*; Zhao, YF. Sensitive and selective offon rhodamine hydrazide fluorescent chemosensor for hypochlorous acid detection and bioimaging, Talanta. 2011, 85, 779-786.
 
37. Huang, ZZ; Lin, L; Gao, Y; Chen, YJ; Yan, XM; Xing, JC; Hang, W*. Bladder cancer determination via two urinary metabolites: a biomarker pattern approach, Mol. Cell. Proteomics. 2011, 10, 10.1074/mcp.M111.007922-1-007922-10.
 
36. Lin, L; Huang, ZZ; Gao, Y; Yan, XM; Xing, JC; Hang, W*. LC-MS based serum metabonomic analysis for renal cell carcinoma diagnosis, staging, and biomarker discovery, J. Proteome. Res., 2011, 10, 1396-1405.
 
35. Zhu, SB; Yang, LL; Long, Y; Gao, M; Huang, TX; Hang, W; Yan, XM*. Size differentiation and absolute quantification of gold nanoparticles via single particle detection with a laboratory-built high-sensitivity flow Cytometer, J. Am. Chem. Soc. 2010, 132, 12176-12178.
 
34. Yang, LL; Wu, LN; Zhu, SB; Long, Y; Hang, W; Yan, XM*. Rapid, absolute, and simultaneous quantification of specific pathogenic strain and total bacterial cells using an ultrasensitive dual-color flow Cytometer, Analytical Chemistry, 2010, 82, 1109-1116.
 
33. Zhang, ZL; Long, Y; Pan, JB; Yan, XM*. Preparation of fluorescence-encoded microspheres in a core-shell structure for suspension arrays, J. Mater. Chem., 2010, 20, 1179-1185.
 
32. Long, Y; Zhang, ZL; Yan, XM*; Xing, JC*; Zhang, KY; Huang, JX; Zheng, JX; Li, W. Multiplex immunodetection of tumor markers with a suspension array built upon core-shell structured functional fluorescence-encoded microspheres, Anal. Chim. Acta, 2010, 665, 63-68.
 
31. Yan, XM; Lin, YM; Huang, RF; Hang, W*; Harrison, W. W. A spectroscopic investigation of the afterglow and recombination process in a microsecond pulsed glow discharge, J. Anal. At. Spectrom. 2010, 25, 534-543.
 
30. Lin, L; Yu, Q; Yan, XM; Hang, W*; Zheng, JX; Xing, JC; Huang, BL. Direct infusion mass spectrometry or liquid chromatography mass spectrometry for human metabonomics? A serum metabonomic study of kidney cancer, Analyst. 2010, 135, 2970-2978.
 
29. Xing, JC*; Yan, LJ; Zhang, J*; Lin, L; Gao, Y; Chen, WG; Song, XY; Yan, XM; Hang, W*; Huang, BL. A comparative study of elution gradients in UPLC-TOF-MS-based metabonomics research, Chromatographia, 2010, 72, 807-813.
 
28. Yang, LL; Zhu, SB; Hang, W; Wu, LN; Yan, XM*. Development of an ultrasensitive dual-channel flow cytometer for the individual analysis of nanosized particles and biomolecules, Anal. Chem., 2009, 81, 2555-2563.
 
27. Ding, J; Lin, L; Hang, W; Yan, XM*. Beryllium uptake and related biological effects studied in THP-1 differentiated macrophages, Metallomics, 2009, 1, 471-478.
 
26. Chen, ZJ; Yan, XM*. Simultaneous determination of melamine and 5-hydroxymethylfurfural in milk by capillary electrophoresis with diode array detection, J. Agric. Food Chem., 2009, 57, 8742-8747.
 
25. Zhang, J*; Yan, LJ; Chen, WG; Lin, L; Song, XY; Yan, XM; Hang, W*; Huang, BL. Metabonomics research of diabetic nephropathy and type 2 diabetes mellitus based on UPLC-oaTOF-MS system, Analytica Chimica Acta, 2009, 650, 16-22.
 
24. Chen, LZ; Lin, L; Yu, Q; Yan, XM; Hang, W*; He, J; Huang, BL. Semiquantitative multielemental analysis of biological samples by a laser ionization orthogonal time-of-flight mass spectrometer, Journal of the American Society for Mass Spectrometry, 2009, 20, 1355-1358.
 
23. Yang, LL; Zhu, SB; Hang, W; Yan, XM*. Development of a single-molecule flow analyzer, Chem. J. Chinese U., 2008, 29, 1549-1551.
 
22. Zhang, J*; Yan, LJ; Lin, L; Chen, WG; Song, XY; Yan, XM; Hang, W*; Huang, BL. Metabonomics research of diabetic mellitus and diabetic nephropathy based on UPLC-oaTOF-MS system, Chemical Journal of Chinese Universities-Chinese, 2008, 29, 2171-2173.
 
21. He, J; Zhong, WW; Tang, AJ; Yan, XM; Lewis, C.; Majidi, V.; Hang, W. A fluorescence detection scheme for ultra large molecules after gas phase separation, Talanta, 2007, 71, 2126-2128.  
 
20. Yan, XM*; Zhong, WW; Tang, AJ; Schielke, E. G.; Hang W, Nolan J. P. Multiplexed flow cytometric immunoassay for influenza virus detection and differentiation, Anal. Chem., 2005, 77, 7673-7678.  
 
19. Yan, XM*; Habbersett R. C.; Yoshida, T. M.; Nolan, J. P.; Jett, J. H.; Marrone, B. L. Probing the kinetics of SYTOX Orange stain binding to double-stranded DNA with implications for DNA analysis, Analytical Chemistry, 2005, 77, 3554-3562.
 
18. Yan, XM*; Tang, AJ; Schielke, E.G.; Hang, W; Nolan, J. P. On the development of a microsphere-based multiplexed immunoassay for influenza virus typing and subtyping by flow cytometry, Options for the control of influenza V, International Congress Series, 2004, 1263, 342-345.
 
17. Yan, XM*; Schielke, E.G.; Grace, K.M.; Hassell, C.; Marrone, B. L.; Nolan, J. P.* Microsphere-based duplexed immunoassay for influenza virus typing by flow cytometry, J. Immunol. Methods2004, 284, 27-38.
 
16. Ferris, M. M.; Yan, XM; Habbersett, R. C.; Shou, YL; Lemanski, C. L.; Jett, J. H.; Yoshida, T. M.; Marrone, B. L. Performance assessment of DNA fragment sizing by high sensitivity flow cytometry and pulsed field gel electrophoresis, J. Clin. Microbiol., 2004, 42, 1965-1976. 
 
15. Yan, XM; Hang, W; Majidi, V.; Marrone, B. L.; Yoshida, T. M. Evaluation of different nucleic acid stains for sensitive dsDNA analysis with capillary electrophoresis separation, J. Chromatogr. A., 2002, 943, 275-285.  
 
14. Yan, XM; Ingeneri, K.; Hang, W; Harrison, W. W. Factors influencing signal profiles in microsecond pulsed glow discharge atomic emission spectrometry, J. Anal. At. Spectrom., 2001, 16, 819-824.
 
13. Yan, XM; Habbersett, R. C.; Cordek, J. M.; Nolan, J. P.; Yoshida, T. M.; Jett, J. H.; Marrone, B. L. Development of a mechanism-based, DNA staining protocol using SYTOX Orange nucleic acid stain and DNA fragment sizing flow cytometry, Anal. Biochem., 2000, 286, 138-148.
 
12. Yan, XM; Grace, W. K.; Yoshida, T. M.; Habbersett, R. C.; Velapan, N.; Jett, J. H.; Keller, R. A.; Marrone, B. L. Characteristics of different nucleic acid staining dyes for DNA fragment sizing by flow cytometry, Anal. Chem., 1999, 71, 5470-5480. 
 
11. Hang, W; Yan, XM; Wayne, D. M.*; Olivares, J. A.; Harrison, W. W.; Majidi, V. Glow discharge source interfacing to mass analyzers: Theoretical and practical considerations, Anal. Chem., 1999, 71, 3231-3237.
 
10. Hang, W; Yan, XM; Wayne, D. M.; Olivares, J. A.; Harrison, W. W.; Majidi, V.* Selective removal of matrix ion peaks in plasma source time-of-flight mass spectrometry: ion deflection and detector gating, J. Anal. At. Spectrom., 1999, 14, 1523-1526.
 
9. Duan, YX; Danen, R. E.; Yan, XM; Steiner, R.; Cuadrado, J.; Wayne, D.; Majidi, V.; Olivares, J. A*. Characterization of an improved thermal ionization cavity source for mass spectrometry, J. Am. Soc. Mass Spectrom., 1999, 10, 1008-1015. 
 
8. Yan, XM; Hang, W; Smith, B. W.; Winefordner, J. D.; Harrison, W. W.* Primary studied of microsecond pulsed glow discharge as an emission sources by using conventional hollow cathode lamp, J. Anal. At. Spectrom., 1998, 13, 1033-1038. 
 
7. Harrison, W. W.*; Hang, W; Yan, XM; Ingeneri, K.; Schilling, C. Temporal considerations with a pulsed glow discharge”, J. Anal. At. Spectrom., 1997, 12, 891-896.
 
6. Yan, XM*; Huang, BL; Tanaka, T.; Kawaguchi, H. Langmuir probe potential measurements for reduced pressure inductively coupled plasma mass spectrometry, J. Anal. At. Spectrom., 1997, 12, 697-701.
 
5. Yan, XM; Tanaka, T.; Kawaguchi, H.* Electrothermal vaporization for the determination of halogens by reduced pressure inductively coupled plasma mass spectrometry, Spectrochim. Acta B, 1996, 51, 1345-1353.
 
4. Yan, XM; Tanaka, T.; Kawaguchi, H.* Reduced pressure inductively coupled plasma mass spectrometry for nonmetallic elements, Appl. Spectrosc., 1996, 50, 182-187. 
 
3. Wang, XR*; Zhuang, ZX; Yang, CL; Yang, PY; Yan, XM; Lin, JM. Development of transient data acquisition system for hyphenated techniques coupled with inductively coupled plasma atomic emission spectrometry, Spectrochim. Acta B, 1996, 51, 1753-1759. 
 
2. Yan, XM; Wang, XR; Yang, PY; Hang, W; Huang, BL*. Signal Acquisition and processing in transient introduction techniques on-line hyphenated with multichannel ICP-AES, Chem. J. Chinese U., 1993, 14, 1506-1509.
 
1. Wang, XR*; Zhu, EY; Yan, XM; Yang, PY; Huang, BL; Zhuang, ZX. Chemometrics study on preliminary clinical diagnostics for cancer patients, Acta Chim. Sinica, 1993, 51, 1094-1098.