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Great progress in Label-Free Virus Analysis

Update: 2017-01-14 13:50      View:

Angew. Chem. Int. Ed. 2016, 55, 10239 –10243
 
 http://dx.doi.org/10.1002/anie.201603007

Great progress was made in the label-free, high-throughput analysis of single viruses by using high-sensitivity flow cytometry, which is the precursor of our product Flow NanoAnalyzer. The paper titled with " Label-Free Analysis of Single Viruses with a Resolution Comparable to That of Electron Microscopy and the Throughput of Flow Cytometry " was online published at Angewandte Chemie International Edition (DOI: 10.1002/anie.201603007), and chosen as Hot Paper.


Viruses are by far the most abundant biological entities on our planet, yet the existing characterization methods are either limited in speed or lack of resolution. By applying a laboratory-built high-sensitivity flow cytometer (HSFCM) to precisely quantify the ultraweak elastically scattered light from single viral particles, here we report a label-free analysis of virus with a resolution comparable to that of electron microscopy and the throughput of flow cytometry. Detection of single viruses down to 27 nm in diameter is demonstrated. T7 and lambda bacteriophages, which differ in size by as little as 4 nm, can be baseline resolved. Moreover, subtle structural differences of the same viral particles can be discriminated. Using monodisperse silica nanoparticles as the size reference standards, the virus sizes measured by the HSFCM agreed with the equivalent particle diameters derived from their structural dimensions. The HSFCM opens a new avenue for virus characterization.