Contact Us
Search

EXTRACELLULAR VESICLES

Update: 2017-01-15 14:12      View:

 

Introduction


Nano-sized extracellular vesicles (EVs) released by various cell types play important roles in a plethora of (patho) physiological processes and are increasingly recognized as biomarkers for disease. In addition, engineered EV and EV-inspired liposomes hold great potential as drug delivery systems. EVs are heterogeneous in composition and size, ranging from approximately 30 to 1000 nm, with the vast majority <200 nm in size. As determined by their biogenesis, the three main classes of EVs are exosomes, microvesicles, and apoptotic bodies. In contrast to microvesicles, which are generated by budding from the plasma membrane, exosomes are derived from the endolysosomal pathway, and fall in the size range of 30-120 nm. Conventional flow cytometry is not able to measure small particles less than 200 nm or dim particles having less than several hundred fluorescent molecules, thus not appropriate for EVs detection. Flow NanoAnalyzer opens a new avenue for single EVs detection. Most of the data hasn’t been published, please keep an eye on the website: www.nanofcm.com.
 

Instrument configuration


The Flow NanoAnalyzer is equipped with a 532 nm CW laser, and the detection channels are side scatter and orange fluorescence (PE).

 

 

Results

   

 

 

Discussion

1.      The SS signal of exosomes from human plasma is detected, and the size distribution analysis of exosomes is achieved by employing silica nanoparticles as size standards.

2.      The concentration of exosomes is measured by fluorescent internal standard method.

3.       Combined with immunofluorescent labeling, the origin and phenotyping of extracellular vesicles can be determined. 

 


  • 上一篇:没有了
  • 下一篇:VIRUS