- Laser (blue, green, red)
- One side-scatter channel
- Two fluorescence channels
- up to 12,000 particles/min
- Lower detection limit 7 nm (gold nanoparticles) to 40 nm (exosomes, viruses)
- High detection limit (1 micron)
Comprehensive LNP Characterization from Cargo to Target Modification Using Nano-Flow Cytometry :
Optimizing RNA encapsulation is vital for formulation development and maximizing delivery to target cells to ensure the success of therapeutics and vaccines. However assessing the efficiency of the optimization process remains challenging. One solution is single particle analyses using technologies like nano-flow cytometry. This technology makes it possible to simultaneously size and count lipid nanoparticles (LNPs) whilst identifying the presence of cargo or surface modifications to determine efficacy of the nanoparticle loading or functionalization.
Date :
Wednesday, July 10, 2024
Time :
8:00 am PT | 11:00 am ET | 17:00 CET
EXTRACELLULAR VESICLES
THE ORIGIN AND PHENOTYPING OF EXTRACELLULAR VESICLES
VACCINE
COMPREHENSIVE MEASUREMENT SUITE FOR NANOSIZED DRUG PRODUCTS
NANOMEDICINE
COMPREHENSIVE MEASUREMENT SUITE FOR NANOSIZED DRUG PRODUCTS
BACTERIA DETECTION
TRULY QUANTITATIVE MEASUREMENT OF TOTAL BACTERIAL TITRE
VIRUSES/VIRAL VACCINE
INTEGRATE INTO THE GENOMES OF THE HOST CELLS
OTHERS
COMPREHENSIVE MEASUREMENT SUITE FOR NANOPARTICLES AND INDIVIDUAL MITOCHONDRIA
EXTRACELLULAR VESICLES
THE ORIGIN AND PHENOTYPING OF EXTRACELLULAR VESICLES
VACCINE
COMPREHENSIVE MEASUREMENT SUITE FOR NANOSIZED DRUG PRODUCTS
NANOMEDICINE
COMPREHENSIVE MEASUREMENT SUITE FOR NANOSIZED DRUG PRODUCTS
BACTERIA DETECTION
TRULY QUANTITATIVE MEASUREMENT OF TOTAL BACTERIAL TITRE
VIRUSES/VIRAL VACCINE
INTEGRATE INTO THE GENOMES OF THE HOST CELLS
OTHERS
COMPREHENSIVE MEASUREMENT SUITE FOR NANOPARTICLES AND INDIVIDUAL MITOCHONDRIA
High Performance
No Swarm Detection
Label Free
Flexible
Particle Concentration
Measure the absolute concentration of both total particles and subpopulations
Phenotyping
Based on single -molecule fluorescence detection, Phenotype quantify direct from marker labeling intensity.
Size Distribution
Particle Concentration
Multiparameter
Phenotyping
For many years scientists yearned for the possibility of performing flow cytometry to analyse small bio-nanoparticles that are too small to be measured by conventional and high sensitivity instruments. These entities, extracellular vesicles, gene therapy vectors, viruses and drug delivery particles, are promised to become the next generation of therapeutics, but they have been hard to handle and characterise due to their small size and biological or chemical heterogeneity. There is therefore a strong case for bringing flow cytometry capability to the sub-200nm scale.
NanoFCM has developed the NanoAnalyzer platform that now enables true flow-cytometry measurement at the sub-micron scale, and down to particle sizes unreachable by any other flow cytometers (10-40nm depending on the nature of the substrate). Nano-flow cytometry, the technology that underpins the NanoAnalyzer, removes bias and uncertainty stemming from the use of fluorescence signal triggering by using its highly sensitive side-scatter channel to trigger particle events. The single-particle nature of the measurement prevents uncontrolled swarming events, reinforcing data integrity. High resolution of both scatter and fluorescence channels allows the assessment of subpopulations, based on size or on bio-chemical properties.
Nano-flow cytometry’s ability to measure simultaneously a (bio)-nanoparticle population for size, size distribution and bio-chemical properties on a single instrument dramatically improves data quality and throughput compared to the traditional, multiple-techniques approach involving particle characterisation and counting (DLS, NTA, RPS), combined with chemical and biological function assessment (ELISA, Western Blot, Flow Cytometry, PCR). Quantitative measurement of the active and contaminant particles in a single preparation opens up the possibility of characterisation-based nanomedicine regulatory approval, and allows the conduct of large-scale clinical studies. From the research laboratory to the quality control department, NanoFCM delivers comprehensive bio-nano analysis.