Nasopharyngeal Carcinoma

Nasopharyngeal carcinoma (NPC) is a malignant tumor commonly associated with Epstein–Barr virus (EBV) infection. Its early diagnosis as well as differentiation from nasopharyngitis (NPG) remain challenging due to the insufficient sensitivity of clinical routine screening methods. Circulating extracellular vesicles (EVs, 40–1000 nm) have shown potential in liquid biopsy for cancer diagnosis and prognosis. Herein, nano-flow cytometry (nFCM) capable of single EV analysis was applied to examine the expression of surface proteins with very low copy numbers on individual EVs as small as 40 nm. The particle concentrations of five EV subsets exposing EBV-encoded latent membrane proteins (LMP1 and LMP2A) and tumor markers (PD-L1, EGFR, and EpCAM) in plasma were determined rapidly via single-particle enumeration. The Flow NanoAnalyzer provides a rapid, reliable and non-invasive method for the diagnosis of NPC, and more accurate screening of NPC, NPG patients and healthy donors in clinical practice.

Figure 1. Detection of five markers of Nasopharyngeal Carcinoma

                           Figure 2. Diagnosis of Nasopharyngeal Carcinoma               Figure 3. Distinction of nasopharyngeal carcinoma,

                                                                                                            NPG patients and healthy donors

Anal. Chem., 2022, 94(27), 9740-9749.