Flow NanoAnalyzer is expected to become a powerful tool for life science, nanoscience and nanotechnology studies. Flow NanoAnalyzer can be used for the multiparameter characterization of natural and synthetic nanoparticles (7-1000 nm) at the single-particle level, such as extracellular vesicles, mitochondria, bacteria, viruses, nanomedicine and nanomaterial. Combining light scattering and fluorescence detection, high-resolution distributions of particle size and biochemical properties can be acquired simultaneously in 1-2 minutes.