Compared with polystyrene beads (RI=1.59), in nFCM system monodisperse silica nanoparticles (RI=1.46) are employed as the reference to calibrate the size of EVs.
The resolution of NanoFCM is comparable to TEM. Median, mean, and standard deviation value of a sample can be read from the software, parameter of gated population within a sample is also available.